It is of greatest significance to have an internal standard for each and every metabolite measured for proper and reproducible metabolic profiling. IROA quantitation kits and IROA internal standard kits are the tools that you can use for metabolic profiling. With IROA quantitation kits, you will be capable of making your own internal standards that are mirroring the metabolome to be measured. These kits comprises of all the reagents that are needed for quantitating, labeling and identifying biochemical components in cell populations productively.
With IROA 100, you will be effectually creating yeast internal standards, can also be identified and quantitate yeast metabolites. With IROA 200, you will e capable of creating bacterial internal standards, identify and quantitate bacterial metabolites. And with IROA 300, you can create mammalian internal standards, identify and quantitate mammalian metabolites. A mammalian metabolic profiling kit includes 13C metabolite yeast extract.
On the other hand, IROA internal standard kits hold complex internal standards that are pre-labelled. It can be used for the identification of hundreds of metabolites across time and equipment. You will attain statistical analysis tools and Cluster Finder software with purchasing of any of the metabolic profiling tools.
In the next few passages of this article, you will know about the IROA metabolic profiling tools that contain 5% and 95% uniformly 13C-labeled yeast extract. Read the points mentioned below in this article properly for exploring more on this topic.
Things you must know about IROA metabolic profiling tools having 13C metabolite yeast extract
IROA has introduced among researchers two quantitation kits for precise mammalian metabolic profiling. This part of this discussion contains some significant factors you need to know about these kits. Read on the following passages attentively for knowing about them fully.
IROA Fluxomic Quantitation Kit has two elements: Labeling Medium, that is liquid and Component Mix, which is dry. The labeling medium comprises of 1 bottle of 250 mL EBSS/RPMI 1640 vitamin solution having 1mg/mL 5% U-13C labeled glucose and 1 empty sterile bottle. You will be able to select media following filter sterilization in it. The component mix includes 657.5mg 5% U-13C metabolite yeast extract and amino acids.
This kit can offer you the reagents to fully label mammalian cells at 5% U-13C. You can consider using these kits in order to decide the flux of a definite precusor through metabolic pathways. The use of 5% will help you in identifying all metabolic tools. This tool also helps in eliminating non-biological signals in an entirely unbiased and non-targeted manner.
IROA Phenotypic Quantitation Kit, this quality IROA kit for mammalian metabolic profiling also includes labeling medium and element mix. The labeling medium contains 1 bottle of 250 mL EBSS/RPMI 1640 vitamin solution including 1mg/mL 95% U-13C labeled glucose and 1 empty sterile bottle. The component mix is 657.5mg 95% U-13C labeled yeast extract and amino acids.
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For finding more details about IROA metabolic profiling tools and 13Cmetabolite yeast extract metabolic standard, you should contact “IROA Technologies LLC”. Visit- iroatech.com, to know more.